|
| Status |
Public on Mar 25, 2009 |
| Title |
NCI-H358_STY |
| Sample type |
genomic |
| |
|
| Source name |
Lung:NSCLC cell line NCI-H358
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Lung
cell line: NCI-H358
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted Qiagen Blood Amp (P/N 51161).
|
| Label |
Biotin
|
| Label protocol |
Labeling and hybridization was performed as described by the 500k assay manual (P/N 701930 Rev. 2)
|
| |
|
| Hybridization protocol |
Labeling and hybridization was performed as described by the 500k assay manual (P/N 701930 Rev. 2)
|
| Scan protocol |
The Arrays were scanned using the Gene Chip Scanner 3000.
|
| Description |
Hybridized to 250K_Sty
|
| Data processing |
CEL files and TXT files (generated by Affymetrix GTYPE using the Dynamic Model Mapping algorithm) were processed by Bioconductor/PLASQ. PLASQ copy number and segmented copy number are included in Sample table. SNP genotype calls are provided as supplementary text files. Call data in the TXT files corresponding to the raw CEL files were generated using GTYPE ver. 4.0 with the Dynamic Model Mapping Algorithm. The threshold for Allele A, Allele B and Allele AB was 0.33.
|
| |
|
| Submission date |
Mar 23, 2009 |
| Last update date |
Mar 24, 2009 |
| Contact name |
Patricia Greninger |
| E-mail(s) |
pgreninger@partners.org
|
| Organization name |
Massachusetts General Hospital Cancer Center
|
| Department |
Center for Molecular Therapeutics
|
| Lab |
Settleman Lab
|
| Street address |
149 13th St
|
| City |
Charlestown |
| State/province |
MA |
| ZIP/Postal code |
02129 |
| Country |
USA |
| |
|
| Platform ID |
GPL3720 |
| Series (1) |
| GSE15126 |
A gene expression signature associated with K-Ras addiction reveals regulators of EMT and tumor cell survival |
|
