|
| Status |
Public on Jun 01, 2021 |
| Title |
FrontalCortex-4_E01 |
| Sample type |
SRA |
| |
|
| Source name |
Brain
|
| Organism |
Macaca mulatta |
| Characteristics |
tissue: FrontalCortex
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were extracted using the Trizol Reagent (Invitrogen, catalog #15596018) and analyzed on an Agilent 2100 Bioanalyzer to access the quality. The quantity of total RNA was measured by Qubit. cDNA was synthesized using the SMARTer PCR cDNA Synthesis Kit (Clotech, catalog #634925) and PrimeSTAR GXL DNA Polymerase (Clontech, catalog #R050B).
SMRT bell libraries were generated by using SMRTbellTM Template prep kit 1.0-SPv3 (PN 100-991-900)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Sequel |
| |
|
| Description |
brain.uniq.sorted.bw
|
| Data processing |
ROI (Reads-Of-Insert) reads were extracted and primer sequences flanking ROI reads were then trimmed
Primer-trimmed reads were further processed to scan the PA tail and trimmed to get PA-trimmed reads which represents reads with a PA tail trimmed.
PA-trimmed reads were then mapped to the reference genome (rheMac8) by GMAP and the low-quality reads were filtered to get the final processed alignments.
Genome_build: rheMac8
Supplementary_files_format_and_content: BigWig files were generated using Iso-seq reads alignment; scores represented Iso-seq reads coverage.
|
| |
|
| Submission date |
Jun 09, 2019 |
| Last update date |
Jun 01, 2021 |
| Contact name |
Shuxian Li |
| E-mail(s) |
1801111861@pku.edu.cn
|
| Phone |
19953859058
|
| Organization name |
Institute of Molecular Medicineļ¼Peking University
|
| Street address |
No.5 Yiheyuan Road Haidian District
|
| City |
Beijing |
| ZIP/Postal code |
100000 |
| Country |
China |
| |
|
| Platform ID |
GPL26751 |
| Series (1) |
| GSE132413 |
Assembly of full-length macaque gene models |
|
| Relations |
| BioSample |
SAMN11997377 |
| SRA |
SRX6027518 |
