|
| Status |
Public on Mar 27, 2009 |
| Title |
Day 7 Chick Liver- Replicate #3 |
| Sample type |
RNA |
| |
|
| Source name |
7 day-old Chick
|
| Organism |
Gallus gallus |
| Characteristics |
tissue: Liver
genotype: Ross broiler chicken
gender: male
age: 7 day-old
|
| Treatment protocol |
Samples of liver, duodenum, breast muscle and whole brain were collected on days 0 and 7 post-hatch, snap frozen in liquid nitrogen and stored at -80C prior to isolation of total RNA.
|
| Growth protocol |
Broiler chicken eggs were hatched and chicks were sampled immediately after emergence from the egg (day 0) and at 7 days post-hatch.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 42C on GeneChip Chicken Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400 using the EukGE-WS2v5_450 script.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GC3000 scanner using factory set PMT settings.
|
| Description |
Gene expression data for liver from 7 day-old broiler chicken.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was set to the default value of 500.
|
| |
|
| Submission date |
Mar 26, 2009 |
| Last update date |
Mar 26, 2009 |
| Contact name |
Mark P Richards |
| E-mail(s) |
Mark.Richards@ars.usda.gov
|
| Phone |
301 504-8892
|
| Fax |
301 504-8623
|
| Organization name |
USDA, ARS
|
| Department |
ANRI
|
| Lab |
Animal Biosciences and Biotechnology Laboratory
|
| Street address |
10300 Baltimore Avenue
|
| City |
Beltsville |
| State/province |
MD |
| ZIP/Postal code |
20705-2350 |
| Country |
USA |
| |
|
| Platform ID |
GPL3213 |
| Series (1) |
| GSE15413 |
Gene expression profiles for different tissues obtained from newly hatched (day 0) and 7 day-old broiler chickens |
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