|
| Status |
Public on Apr 04, 2020 |
| Title |
S342: SC_etoh_rep2 |
| Sample type |
SRA |
| |
|
| Source name |
A549
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: A549
cell type: scramble
treatment: ethanol
|
| Treatment protocol |
100nM Dex or equal volume ethanol for 24 hours
|
| Growth protocol |
Cultured in DMEM supplemented with 10% FBS, and changed to DMEM with 5% charcoal stripped FBS for 24 hours prior to Dex or ethanol treatment
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Trizol
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Data processing |
Salmon quantification produced count data
DEseq2 differential expression analysis produced p-values and log2 fold change values
|
| |
|
| Submission date |
Jun 10, 2019 |
| Last update date |
Apr 04, 2020 |
| Contact name |
Feizhen Wu |
| E-mail(s) |
wufz@fudan.edu.cn
|
| Phone |
86-21-54237821
|
| Organization name |
Fudan Univ, Shanghai, China
|
| Department |
Institutes of Biomedical Sciences
|
| Lab |
Epigenetics lab
|
| Street address |
Dongan Road 131, Rm 511 Mingdao Building
|
| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
| |
|
| Platform ID |
GPL20795 |
| Series (1) |
| GSE108588 |
GR and LSD1/KDM1A-targeted gene activation requires selective H3K4me2 demethylation at enhancers |
|
| Relations |
| BioSample |
SAMN12009277 |
| SRA |
SRX6035625 |
