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Status |
Public on Aug 08, 2019 |
Title |
β-catenin_std_Day 2-1 |
Sample type |
SRA |
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Source name |
Human iPS-derived cells on day 2 of the standard 2C protocol
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Organism |
Homo sapiens |
Characteristics |
cell type: Human iPS cells tissue: Dermal fibroblasts days after differentiation: 2 antibody: Rabbit anti-non-phospho β-catenin antibody (Cell Signaling Technology, 8814)
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Treatment protocol |
Human iPS cells were differentiated to chondrocytes by the standard or altered 2C differentiation protocols.
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Extracted molecule |
genomic DNA |
Extraction protocol |
For ATAC-seq, 50,000 cells were lysed to obtain nuclei in cold lysis buffer, and incubated in the Tn5 transposase reaction mix, as described previously (Buenrostro et al., 2013). For ChIP-seq, cells were cross-linked with 1% formaldehyde, and after lysis of cells, chromatin was fragmented by sonication. Protein-DNA complexes were purified with antibody. For ATAC-seq, DNA fragments were PCR-amplified using previously-designed barcoded primers, as described previously (Buenrostro et al., 2013).For ChIP-seq, libraries were constructed with ThruPLEX DNA-seq Kit (Takara Bio), according to manufacturer’s instruction.
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Library strategy |
ChIP-Seq |
Library source |
genomic |
Library selection |
ChIP |
Instrument model |
HiSeq X Ten |
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Data processing |
DNA-sequence information was aligned to the human genome reference sequence (hg19) by bowtie aligner (Langmead et al., 2009). Peak calling was performed by two-sample analysis on CisGenome software (Ji et al., 2008) with a P-value cutoff of 10-5 comparing with the input control. Genome_build: hg19 Supplementary_files_format_and_content: .bed file reports peaks
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Submission date |
Jun 11, 2019 |
Last update date |
Aug 09, 2019 |
Contact name |
Manabu Kawata |
E-mail(s) |
manabukawata@hotmail.com
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Organization name |
The University of Tokyo
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Street address |
7-3-1 Hongo
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City |
Bunkyo-ku |
State/province |
Tokyo |
ZIP/Postal code |
113-0033 |
Country |
Japan |
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Platform ID |
GPL20795 |
Series (1) |
GSE132532 |
β-catenin ChIP-seq, RARα ChIP-seq, and ATAC-seq datasets in the standard or altered 2C protocols. |
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Relations |
BioSample |
SAMN12018384 |
SRA |
SRX6039031 |
Supplementary file |
Size |
Download |
File type/resource |
GSM3873119_b-catenin_std_D2-1_ChIP.2s.FDR1.peaks.bed.gz |
34.4 Kb |
(ftp)(http) |
BED |
SRA Run Selector |
Raw data are available in SRA |
Processed data provided as supplementary file |
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