|
| Status |
Public on Sep 24, 2019 |
| Title |
KO.zygote.rep2 |
| Sample type |
SRA |
| |
|
| Source name |
Embryos
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype: Dux knockout
developmental stage: zygote
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNAs in embryos lysis with a polyadenylated tail were captured, reverse transcripted and preamplified following the protocol for Smart-seq2 sequence library generation
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
Gene Abundance
|
| Data processing |
reads( >35bp) were then aligned to the mouse genome (mm9) using STAR
all Refseq genes were quantified to FPKM using Stringtie
Deseq2 was used to perform differential analysis
Genome_build: mm9
Supplementary_files_format_and_content: fpkm files were quantified using Stringtie
|
| |
|
| Submission date |
Jun 11, 2019 |
| Last update date |
Sep 25, 2019 |
| Contact name |
Mingyue Guo |
| E-mail(s) |
1610815@tongji.edu.cn
|
| Phone |
86-21-65982278
|
| Organization name |
Tongji University
|
| Department |
School of Life science and technology
|
| Lab |
Gao Shaorong
|
| Street address |
Siping
|
| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
200092 |
| Country |
China |
| |
|
| Platform ID |
GPL24247 |
| Series (2) |
| GSE132045 |
Single cell RNA-seq Analysis of Wild Type(WT), Dux knockout(KO) and Dux overexpression(OE) embryos |
| GSE134832 |
Precise temporal regulation of Dux is important for embryo development |
|
| Relations |
| BioSample |
SAMN12026824 |
| SRA |
SRX6045881 |
