|
| Status |
Public on Oct 29, 2009 |
| Title |
cultured epiblast cEpi#2 |
| Sample type |
RNA |
| |
|
| Source name |
cultured epiblast
|
| Organism |
Mus musculus |
| Characteristics |
cell type: cultured epiblast
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to manufacturer's instructions
|
| Label |
digoxigenin
|
| Label protocol |
Digoxigenin labeled cRNA was prepared according to Applied Biosystems nanoamp RT-IVT protocol
|
| |
|
| Hybridization protocol |
Hybridization was performed according to Applied Biosystems Chemiluminescence Detection Kit Protocol (PN 4339627) using 10 ug fragmented cRNA
|
| Scan protocol |
Image was captured using CCD camera came with AB1700 Microarray Analyzer
|
| Description |
Epiblast from E5.5-E7.5 postimplantation embryos obtained as described above, was dissected with glass needles based on morphology, and treated with EGTA in PBS for 10min, followed by trypsin for 5min at room temperature.
Gene expression data from mouse embyronic cells using Applied Biosystems AB1700 Whole Genome Microarray
|
| Data processing |
Quantile normalization was performed. The normalization was performed using ABarray bioconductor package
|
| |
|
| Submission date |
Mar 31, 2009 |
| Last update date |
Oct 29, 2009 |
| Contact name |
Fuchou Tang |
| Organization name |
University of Cambridge
|
| Department |
Wellcome Trust/Cancer Research UK Gurdon Institute
|
| Street address |
Tennis Court Road
|
| City |
Cambridge |
| State/province |
Cambridgeshire |
| ZIP/Postal code |
CB2 1QN |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL2995 |
| Series (1) |
| GSE15487 |
Breaking the epigenetic barrier during reversion of post-implantation epiblast cells |
|
