|
| Status |
Public on Sep 03, 2009 |
| Title |
06R030 RA |
| Sample type |
RNA |
| |
|
| Source name |
Peripheral Mononuclear Blood Cells
|
| Organism |
Homo sapiens |
| Characteristics |
status: Rheumatoid Arthritis Patient
gender: Female
age: 54
tissue: Blood
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from Peripheral Blood using the PAXgene RNA isolation kit (PreAnalytix, GmbH, Germany). Total RNA yield (ng) was determined spectrophotometrically using the NanoDrop ND-1000 (Wilmington). Total RNA profile was determined using the Bioanalyzer 2100 (Agilent).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion Illumina TotalPrep RNA Amplification kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
none
|
| Data processing |
The data were normalised using quantile normalisation with Beadstudio
|
| |
|
| Submission date |
Apr 06, 2009 |
| Last update date |
Sep 03, 2009 |
| Contact name |
Robert Olaso |
| E-mail(s) |
olaso@cng.fr
|
| Phone |
0000000000
|
| Fax |
0000000000
|
| Organization name |
CEA IG
|
| Department |
CNG Technology Department
|
| Lab |
Gene Expression Platform
|
| Street address |
2 rue gaston cremieux
|
| City |
Evry |
| ZIP/Postal code |
91057 |
| Country |
France |
| |
|
| Platform ID |
GPL6102 |
| Series (1) |
| GSE15573 |
Immunity and Defense Genes in Peripheral Blood Mononuclear Cells of Rheumatoid Arthritis patients |
|
