|
| Status |
Public on Jun 18, 2022 |
| Title |
NEK9_MCF7_5 |
| Sample type |
SRA |
| |
|
| Source name |
breast
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MCF7
sirna: NEK9
replicate: 5
|
| Treatment protocol |
Cells were treateded with SMARTpool siRNA targeting the gene of interest as per the manufactures protocol.
|
| Growth protocol |
Cells were grown in DMEM +10%FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted by RNeasy mini kit
TruSeq RNA Library Prep Kit
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
Knockdown of NEK9 in MCF7 cells
MCF7.txt
|
| Data processing |
Align with hisat2
Establish count matrix with Rsubread
Genome_build: hg19
Supplementary_files_format_and_content: CSV file containg matrix of read counts for individual genes. Genes are stored by Entrez Gene ID.
|
| |
|
| Submission date |
Jun 19, 2019 |
| Last update date |
Jun 18, 2022 |
| Contact name |
Andrew Nicholas Holding |
| E-mail(s) |
andrew.holding@york.ac.uk
|
| Organization name |
University of York
|
| Department |
Biology
|
| Lab |
Holding Lab
|
| Street address |
Wentwork Way
|
| City |
York |
| ZIP/Postal code |
YO10 5DD |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL11154 |
| Series (1) |
| GSE133012 |
Measuring the transcriptomic response to targeting kinases correlated with estrogen receptor activity in Breast Cancer |
|
| Relations |
| BioSample |
SAMN12096388 |
| SRA |
SRX6095372 |
