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| Status |
Public on Jun 21, 2022 |
| Title |
WAT: negative control 2 (NC2) |
| Sample type |
SRA |
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| Source name |
Subcutaneous WAT
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| Organism |
Mus musculus |
| Characteristics |
tissue: white adipose tissue (WAT)
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| Extracted molecule |
total RNA |
| Extraction protocol |
Adipose tissues were collected, flash frozen on dry ice, and RNA was harvested using Trizol reagent. NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
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| Data processing |
Reads were aligned to mm10, using Tophat (version tophat-2.0.11) and genes were quantified using Cufflinks (Version 2.1.1)
Genome_build: mm10
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| Submission date |
Jun 21, 2019 |
| Last update date |
Jun 21, 2022 |
| Contact name |
Lei Sun |
| E-mail(s) |
sun.lei@duke-nus.edu.sg
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| Organization name |
Duke-NUS
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| Street address |
8 College Road
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| City |
Singapore |
| ZIP/Postal code |
169857 |
| Country |
Singapore |
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| Platform ID |
GPL13112 |
| Series (1) |
| GSE133142 |
cAMP-MicroRNA-203-IFNγ Network Regulates Subcutaneous White Fat Browning and Glucose Tolerance |
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| Relations |
| BioSample |
SAMN12107334 |
| SRA |
SRX6102428 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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