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Sample GSM3912987 Query DataSets for GSM3912987
Status Public on Jul 02, 2019
Title MDBK-GFP
Sample type SRA
 
Source name MDBK cells expressing GFP
Organism Bos taurus
Characteristics tissue: Kidney
cell line: MDBK
cell type: epithelial cells
Extracted molecule total RNA
Extraction protocol Ambion mirVana miRNA Isolation Kit (Thermo Fisher Scientific)
TruSeq Small RNA Sample Prep Kits(Illumina, San Diego, USA)
 
Library strategy miRNA-Seq
Library source transcriptomic
Library selection size fractionation
Instrument model Illumina HiSeq 2500
 
Data processing Single-end sequencing on Illumina HiSeq 2500
Removing low quality reads containing more than one low quality (Q-value≤20) base or containing unknown nucleotides(N);
Removing reads containing adapters
Removing reads shorter than 18nt (not include adapters).
genome build: GCA_000003055.3
Differentially expressed miRNAs were identified with fold change ≥2 and P value <0.05.
Supplementary_files_format_and_content: MDBK-GFP-E5 vs MDBK-GFP miRNA differential expression
 
Submission date Jul 01, 2019
Last update date Jul 07, 2019
Contact name Feng Pang
E-mail(s) 19871122asdf@163.com
Organization name Hainan University
Street address Renmin Road 58
City Haikou
ZIP/Postal code 570228
Country China
 
Platform ID GPL19172
Series (2)
GSE133610 Alterations of miRNAs in MDBK cells expressing BPV-13 E5
GSE133614 Alterations of miRNAs and mRNAs in MDBK cells expressing BPV-13 E5
Relations
BioSample SAMN12172981
SRA SRX6385627

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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