|
Status |
Public on Jul 02, 2019 |
Title |
MDBK-GFP |
Sample type |
SRA |
|
|
Source name |
MDBK cells expressing GFP
|
Organism |
Bos taurus |
Characteristics |
tissue: Kidney cell line: MDBK cell type: epithelial cells
|
Extracted molecule |
total RNA |
Extraction protocol |
Ambion mirVana miRNA Isolation Kit (Thermo Fisher Scientific) TruSeq Small RNA Sample Prep Kits(Illumina, San Diego, USA)
|
|
|
Library strategy |
miRNA-Seq |
Library source |
transcriptomic |
Library selection |
size fractionation |
Instrument model |
Illumina HiSeq 2500 |
|
|
Data processing |
Single-end sequencing on Illumina HiSeq 2500 Removing low quality reads containing more than one low quality (Q-value≤20) base or containing unknown nucleotides(N); Removing reads containing adapters Removing reads shorter than 18nt (not include adapters). genome build: GCA_000003055.3 Differentially expressed miRNAs were identified with fold change ≥2 and P value <0.05. Supplementary_files_format_and_content: MDBK-GFP-E5 vs MDBK-GFP miRNA differential expression
|
|
|
Submission date |
Jul 01, 2019 |
Last update date |
Jul 07, 2019 |
Contact name |
Feng Pang |
E-mail(s) |
19871122asdf@163.com
|
Organization name |
Hainan University
|
Street address |
Renmin Road 58
|
City |
Haikou |
ZIP/Postal code |
570228 |
Country |
China |
|
|
Platform ID |
GPL19172 |
Series (2) |
GSE133610 |
Alterations of miRNAs in MDBK cells expressing BPV-13 E5 |
GSE133614 |
Alterations of miRNAs and mRNAs in MDBK cells expressing BPV-13 E5 |
|
Relations |
BioSample |
SAMN12172981 |
SRA |
SRX6385627 |