|
| Status |
Public on Jul 02, 2019 |
| Title |
siNSUN2-T_rep1 |
| Sample type |
SRA |
| |
|
| Source name |
T24 cell line
|
| Organism |
Homo sapiens |
| Characteristics |
strain: T24 cell line
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Total RNA was isolated using TRIzol® Reagent (Ambion). The RNA concentration and quality were determined with NanoDrop, Qubit and agarose gel analysis. The Dynabeads® mRNA Purification Kit (Ambion) was used to enrich the mRNAs, which were used as templates for RNA-Seq library construction.
RNA-Seq libraries was directly constructed by using KAPA mRNA Stranded mRNA-Seq Kit (KAPA) according to the manufacturer’s instructions. The libraries were sequenced on the Illumina HiSeq X-Ten platform at Novogene (Tianjin, CA) with paired-end 150 bp read length.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Description |
RNAseq of siNSUN2 replicate 1 in T24 cell
|
| Data processing |
Reads were mapped to the hg19 genome with hisat2.
The number of reads mapped to each Ensembl gene was counted using HTSeq
Genome_build: hg19
Supplementary_files_format_and_content: txt, Reads count for each sample.
|
| |
|
| Submission date |
Jul 01, 2019 |
| Last update date |
Jul 07, 2019 |
| Contact name |
Bao-Fa Sun |
| E-mail(s) |
sunbf@big.ac.cn
|
| Organization name |
Beijing Institute of Genomics (BIG) of Chinese Academy of Sciences (CAS)
|
| Street address |
Da-Tun Road
|
| City |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
| |
|
| Platform ID |
GPL20795 |
| Series (2) |
| GSE133621 |
Transcriptomics analysis of gene expression in wildtype and NSUN2 knockdown T24 cell |
| GSE133671 |
m5C Promotes Pathogenesis of the Bladder Cancer Through Stabilizing mRNAs |
|
| Relations |
| BioSample |
SAMN12173572 |
| SRA |
SRX6385979 |
