|
| Status |
Public on Aug 05, 2020 |
| Title |
Leptin treated mouse 4 |
| Sample type |
RNA |
| |
|
| Source name |
ob/ob leptin treated islets, biological replicate 4
|
| Organism |
Mus musculus |
| Characteristics |
tissue: pancreatic islets
gender: male
agent: leptin
|
| Treatment protocol |
Pancreatic islets were isolated from ob/ob mice, 21 days after leptin or saline (Ctl) infusion.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extracted with miRNeasy kit QIAGEN following manufacturer's instructions
|
| Label |
cyanine3
|
| Label protocol |
100ng of total RNA was submitted to microRNA Labeling & Hybridization Kit
|
| |
|
| Hybridization protocol |
hybridization was performed according to microRNA Labeling & Hybridization Kit
|
| Scan protocol |
After hybridization and washing the arrays were scanned with an Agilent microarray scanner G2505B using high dynamic range settings as specified by the manufacturer.
|
| Data processing |
Agilent Feature Extraction Software v 10.7.3.1 was used to extract the data. Quantile normalization was applied to the Total Gene Signal intensity values, using the function normalize.quantiles from the R Bioconductor package preprocessCore.
|
| |
|
| Submission date |
Jul 05, 2019 |
| Last update date |
Aug 05, 2020 |
| Contact name |
Claudiane Guay |
| E-mail(s) |
claudiane.guay@unil.ch
|
| Phone |
+41216925281
|
| Organization name |
University of Lausanne
|
| Department |
DNF
|
| Street address |
Du Bugnon 9
|
| City |
Lausanne |
| State/province |
Vaud |
| ZIP/Postal code |
1005 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL21265 |
| Series (2) |
| GSE133851 |
Effects of leptin supplementation on pancreatic islet miRNA expression in ob/ob mice |
| GSE133852 |
ob/ob mice on pancreatic islet |
|
