|
| Status |
Public on Jun 02, 2009 |
| Title |
gra1-D, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
gra1-D mutant
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
ecotype: Col-0
tissue: vegetative part
age: stage 1.03
genotype: gra1-D
|
| Growth protocol |
plant were grown on MS medium under 16 h light/ 8 h dark cycle at 22°C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy plant mini kit (Qiagen) , according to manufacturer protocol
|
| Label |
biotin
|
| Label protocol |
Standard affymetrix protocol
|
| |
|
| Hybridization protocol |
Standard affymetrix protocol
|
| Scan protocol |
Standard affymetrix protocol
|
| Description |
gra1-D mutant, vegetative part of seedlings at stage 1.03
|
| Data processing |
Expression data were processed using RMA (background correction, normalization and summarization) in BioConductor (Gentleman et al. 2004; Irizarry et al. 2003a; 2003b). An alternative CDF (tinesath1cdf) was used, where each probe is assigned uniquely to one transcript and is excluded if it aligns to a different transcript with 24 or more perfect matches (Casneuf et al. 2007; http://www.bioconductor.org/packages/release/data/experiment/html/tinesath1cdf.html)
|
| |
|
| Submission date |
Apr 17, 2009 |
| Last update date |
Jun 02, 2009 |
| Contact name |
Nathalie Gonzalez |
| E-mail(s) |
nagon@psb.vib-ugent.be
|
| Phone |
+32 (0)9 331 39 56
|
| Organization name |
VIB
|
| Department |
Plant System Biology
|
| Lab |
System Biology of Yield
|
| Street address |
Technologiepark 927
|
| City |
Gent |
| ZIP/Postal code |
9052 |
| Country |
Belgium |
| |
|
| Platform ID |
GPL8455 |
| Series (2) |
| GSE15722 |
Chromosomal duplication in the grandifolia-D mutants of Arabidopsis thaliana, expression data |
| GSE15726 |
Chromosomal duplication in the grandifolia-D mutants of Arabidopsis thaliana |
|
