|
| Status |
Public on Jul 31, 2019 |
| Title |
02_R2-Wild_Type_SX1263 |
| Sample type |
SRA |
| |
|
| Source name |
Pmex-5::gfp::h2b::tbb-2 (mjIs134 II)
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
strain: SX1263
genotype: Pmex-5::gfp::h2b::tbb-2 (mjIs134 II)
tissue: Dissected Gonads
temperature: 20 C
|
| Growth protocol |
20C fed on E. coli OP-50 bacteria.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
miRNeasy Kit (Qiagen)
NEBNext® Multiplex Small RNA Library Prep Set for Illumina®.
|
| |
|
| Library strategy |
ncRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
Small RNA-seq
|
| Data processing |
Adapter trimming - cutadapt
Quality examination - fastQC
Alignment - Shortstack
Filter-20-23 bp
Counting - HTseq_count
Comparisons - DESeq2
Genome_build: WS235/ce11
Supplementary_files_format_and_content: *.count.txt: HTseq_count output files.
|
| |
|
| Submission date |
Jul 09, 2019 |
| Last update date |
Aug 02, 2019 |
| Contact name |
Itai Antoine Toker |
| E-mail(s) |
itaitoker@gmail.com
|
| Organization name |
Tel Aviv University
|
| Department |
Neurobiology
|
| Lab |
Oded Rechavi lab
|
| Street address |
Haim levanon St.
|
| City |
Tel Aviv |
| ZIP/Postal code |
69978 |
| Country |
Israel |
| |
|
| Platform ID |
GPL19757 |
| Series (1) |
| GSE128112 |
Germ Granules Govern Small RNA Inheritance |
|
| Relations |
| BioSample |
SAMN12240143 |
| SRA |
SRX6423854 |
