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| Status |
Public on Jul 12, 2019 |
| Title |
BHK |
| Sample type |
SRA |
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| Source name |
BHK-21
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| Organism |
Mesocricetus auratus |
| Characteristics |
cell type: BHK-21
infection: none
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| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Trizol reagent. Illumina TruSeq™ Small RNA Sample Prep Kits(Illumina, San Diego, USA) was used for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
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| Data processing |
the raw reads were subjected to the Illumina pipeline filter (Solexa 0.3)
Subsequently, unique sequences with length in 18~26 nucleotide were mapped to specific species precursors in miRBase 20.0 by BLAST search to identify known miRNAs and novel 3p- and 5p- derived miRNAs.
Length variation at both 3’ and 5’ ends and one mismatch inside of the sequence were allowed in the alignment.
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| Submission date |
Jul 11, 2019 |
| Last update date |
Jul 12, 2019 |
| Contact name |
Tu zhong zhong |
| E-mail(s) |
tudong890901@163.com
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| Phone |
13596046471
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| Organization name |
CVRI
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| Street address |
LIUYING XILU 666
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| City |
CHANGCHUN CITY |
| ZIP/Postal code |
130122 |
| Country |
China |
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| Platform ID |
GPL22399 |
| Series (1) |
| GSE134156 |
High-throughput sequencing of MiRNA |
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| Relations |
| BioSample |
SAMN12255756 |
| SRA |
SRX6431539 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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