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Sample GSM397308 Query DataSets for GSM397308
Status Public on May 01, 2010
Title BCR/ABL1 biological replicate 2
Sample type RNA
 
Source name Human CD34+ cord blood cells expressing BCR/ABL1
Organism Homo sapiens
Characteristics cell source: Human CD34+ cells isolated from umbilical cord blood
agent: BCR/ABL1
biological replicate: 2
Extracted molecule total RNA
Extraction protocol RNA was isolated using the RNeasy isolation kit (Qiagen, Valencia, CA) according to the manufacturers’ instructions. RNA quality was assessed using a Bioanalyzer (Agilent Technologies, Inc., Santa Clara, CA).
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Gene Expression Analysis Technical Manual 701021 rev.5).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Gene Chip Human Gene U133 plus 2.0 microarrays. GeneChips were washed and stained in the Affymetrix Fluidics station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner GSC3000.
Description Gene expression profile of human CD34+ cord blood cells expressing BCR/ABL1
Data processing Normalization was performed using the RMA method in Gene Chip Operating Software and the obtained data matrix was imported into the TIGR Multi Experiment Viewer for further analysis.
 
Submission date Apr 24, 2009
Last update date Aug 28, 2018
Contact name Helena Ă…gerstam
Organization name Lund University
Street address Lund University Hospital
City Lund
ZIP/Postal code SE-22158
Country Sweden
 
Platform ID GPL570
Series (1)
GSE15811 ZMYM2/FGFR1, BCR/FGFR1 or BCR/ABL1 in human cord blood CD34+ cells reveals similar but distinct gene expression profiles
Relations
Reanalyzed by GSE119087

Data table header descriptions
ID_REF
VALUE Quantification

Data table
ID_REF VALUE
AFFX-BioB-5_at 7.98879
AFFX-BioB-M_at 8.13392
AFFX-BioB-3_at 7.75006
AFFX-BioC-5_at 7.97887
AFFX-BioC-3_at 8.22606
AFFX-BioDn-5_at 11.0327
AFFX-BioDn-3_at 11.8774
AFFX-CreX-5_at 13.3367
AFFX-CreX-3_at 13.8208
AFFX-DapX-5_at 8.13281
AFFX-DapX-M_at 9.00817
AFFX-DapX-3_at 9.62402
AFFX-LysX-5_at 4.58492
AFFX-LysX-M_at 5.43551
AFFX-LysX-3_at 6.53746
AFFX-PheX-5_at 5.58334
AFFX-PheX-M_at 6.0067
AFFX-PheX-3_at 6.32539
AFFX-ThrX-5_at 6.50364
AFFX-ThrX-M_at 7.07998

Total number of rows: 54675

Table truncated, full table size 998 Kbytes.




Supplementary file Size Download File type/resource
GSM397308.CEL.gz 4.6 Mb (ftp)(http) CEL
GSM397308.chp.gz 373.7 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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