|
| Status |
Public on May 01, 2010 |
| Title |
BCR/FGFR1 biological replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
Human CD34+ cord blood cells expressing BCR/FGFR1
|
| Organism |
Homo sapiens |
| Characteristics |
cell source: Human CD34+ cells isolated from umbilical cord blood
agent: BCR/FGFR1
biological replicate: 3
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using the RNeasy isolation kit (Qiagen, Valencia, CA) according to the manufacturers’ instructions. RNA quality was assessed using a Bioanalyzer (Agilent Technologies, Inc., Santa Clara, CA).
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Gene Expression Analysis Technical Manual 701021 rev.5).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Gene Chip Human Gene U133 plus 2.0 microarrays. GeneChips were washed and stained in the Affymetrix Fluidics station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner GSC3000.
|
| Description |
Gene expression profile of human CD34+ cord blood cells expressing BCR/FGFR1
|
| Data processing |
Normalization was performed using the RMA method in Gene Chip Operating Software and the obtained data matrix was imported into the TIGR Multi Experiment Viewer for further analysis.
|
| |
|
| Submission date |
Apr 24, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Helena Ågerstam |
| Organization name |
Lund University
|
| Street address |
Lund University Hospital
|
| City |
Lund |
| ZIP/Postal code |
SE-22158 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE15811 |
ZMYM2/FGFR1, BCR/FGFR1 or BCR/ABL1 in human cord blood CD34+ cells reveals similar but distinct gene expression profiles |
|
| Relations |
| Reanalyzed by |
GSE119087 |
