Monocytes were isolated from 30 ml of whole blood from 19 women, 10 with high BMD and 9 with low BMD, using monocyte negative isolation kit from Dynal Biotech Inc. Total RNA was extracted from monocytes using Qiagen RNeasy Mini Kit. Targets were produced for each subject using standard Affymetrix procedures from about 4ug of total RNA. Hybridization was made for each subject. Comparison was performed between 10 high BMD and 9 low BMD subjects. MCI-003 is the sample from a premenopausal high BMD subjects. Keywords = microarray Keywords = monocyte Keywords = osteoporosis Keywords = HDC Keywords = CCR3 Keywords = GCR
