|
| Status |
Public on Apr 20, 2010 |
| Title |
MDA-MB-231 breast cell line subpopulation expressing CXCR4 treated with SDF-1alpha 1 |
| Sample type |
RNA |
| |
|
| Source name |
MDA-MB-231 cell line
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Breast cancer cell line
cell line: MDA-MB-231
cell population: CXCR4 positive
treatment: SDF1
|
| Treatment protocol |
Cells were treated with SDF-1alpha (400ng/ml) for 1 hour or were left untreated.
|
| Growth protocol |
Cells were maintained in Minimum Essentail Media with 10% fetal calf serum, penecilin/streptomycin, and 1 nM Insulin. Cells were cultured for three days after sorting.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen Rneasy
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the Affymetrix standard single cycle 3' IVT protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C onto Human HGU133 plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
Scanned using Affymetrix GeneChip® Scanner 3000 7G
|
| Description |
none
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
|
| |
|
| Submission date |
Apr 29, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Harikrishna Nakshatri |
| Organization name |
Indiana University School of Medicine
|
| Department |
General Surgery
|
| Street address |
980 W. Walnut St. R3C218C
|
| City |
Indianapolis |
| State/province |
IN |
| ZIP/Postal code |
46202 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE15893 |
Gene expression pattern in CXCR4+ and CXCR4- subpopulation of breast cancer cells |
|
| Relations |
| Reanalyzed by |
GSE119087 |
