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Sample GSM399362 Query DataSets for GSM399362
Status Public on May 02, 2009
Title T.4+8int.Th#1
Sample type RNA
 
Source name thymus
Organism Mus musculus
Characteristics age: 6 weeks
exclusion markers: 11b- 11c- 19- 49b- Gr1- TER119-
genetic background: C57BL/6J
phenotype markers: 4+ 8int TCRhi
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol Glass Slide Flow Sorting
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description T.4+8int.Th#1, Positive Selection, Intermediate
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Apr 30, 2009
Last update date May 24, 2016
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 19.8684
10344622 313.1524
10344624 321.6448
10344633 843.8224
10344637 201.9093
10344653 18.4744
10344658 299.2957
10344674 14.9426
10344679 48.9647
10344707 301.6492
10344713 226.47
10344723 173.7832
10344725 47.1241
10344741 2679.4799
10344743 28.5938
10344750 599.087
10344772 27.572
10344789 224.9788
10344797 424.1357
10344799 251.3324

Total number of rows: 24922

Table truncated, full table size 422 Kbytes.




Supplementary file Size Download File type/resource
GSM399362_EA07068_56648_MoGene_T.4+8int.Th_#1.cel.gz 3.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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