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Sample GSM399396 Query DataSets for GSM399396
Status Public on May 02, 2009
Title T.DP69+.Th#3
Sample type RNA
 
Source name thymus
Organism Mus musculus
Characteristics age: 6 weeks
exclusion markers: 11b- 11c- 19- 49b- Gr1- TER119-
genetic background: C57BL/6J
phenotype markers: 4+ 8+ TCRlo/int 69+
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol Glass slide teasing Flow sorting
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description T.DP69+.Th#3, Double-Positive, Early Positive Selection
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Apr 30, 2009
Last update date May 24, 2016
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 18.3015
10344622 255.8794
10344624 374.6962
10344633 706.4045
10344637 238.0187
10344653 27.1568
10344658 141.4731
10344674 21.523
10344679 42.4415
10344707 164.4913
10344713 284.018
10344723 253.6531
10344725 58.8646
10344741 2469.3059
10344743 30.2628
10344750 369.992
10344772 34.2178
10344789 167.4892
10344797 547.4257
10344799 130.9754

Total number of rows: 24922

Table truncated, full table size 422 Kbytes.




Supplementary file Size Download File type/resource
GSM399396_EA07068_52767_MoGene_T.DP69+.Th_#3.cel.gz 3.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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