|
Status |
Public on Jul 23, 2022 |
Title |
H1975-plx304_siMESH1-01_72h-rep1 |
Sample type |
RNA |
|
|
Source name |
H1975 cells
|
Organism |
Homo sapiens |
Characteristics |
cell line: H1975 transfection: plx304 vector and reverse transfected with the siRNA targeting MESH1 CDS (siMESH1) for 72h
|
Treatment protocol |
H1975 cells were reverse transfected with the corresponding siRNA together with the Lipofectamine RNAiMAX reagent (ThermoFisher) and Opti-MEM according to the manufacture's instructions.
|
Growth protocol |
All H1975 cells were cultured in the DMEM media supplement with 10% FBS and Penicillin/Streptomycin
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted by RNAeasy kits (Qiagen) according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Standard Affymetrix protocol
|
|
|
Hybridization protocol |
Hybridization targets were prepared with MessageAmp™ Premier RNA Amplification Kit (Applied Biosystems/Ambion, Austin, TX) from total RNA, hybridized to GeneChip arrays in Affymetrix GeneChip® hybridization oven 645, washed in Affymetrix GeneChip® Fluidics Station 450
|
Scan protocol |
scanned with Affymetrix GeneChip® Scanner 7G according to standard Affymetrix GeneChip® Hybridization, Wash, and Stain protocols. (Affymetrix, Santa Clara,CA).
|
Data processing |
The data were analyzed with Agilent 2100 Bioanalyzer G2939A
|
|
|
Submission date |
Aug 04, 2019 |
Last update date |
Jul 24, 2022 |
Contact name |
Jen-Tsan Chi |
E-mail(s) |
jentsan.chi@duke.edu
|
Phone |
919-668-4759
|
Organization name |
Duke University
|
Department |
Molecular Genetics and Microbiology
|
Street address |
101 Science Drive, DUMC 3382
|
City |
Durham |
State/province |
NC |
ZIP/Postal code |
27708 |
Country |
USA |
|
|
Platform ID |
GPL571 |
Series (1) |
GSE135346 |
TAZ overexpression partially rescued the transcriptomic reprogramming triggered by MESH1 silencing |
|