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Sample GSM400676 Query DataSets for GSM400676
Status Public on May 13, 2009
Title Day1-RT2-ArrayA
Sample type RNA
 
Source name Proliferating C2C12 cells
Organism Mus musculus
Characteristics tissue: muscle
strain: C3H
cell type: myoblast
time: day 1
time: aliquot 2
Biomaterial provider ATCC
Treatment protocol N/A
Growth protocol Mouse C2C12 myoblasts were cultured in growth media containing Dulbecco's Modified Eagle Medium (DMEM) (ATCC), supplemented with 10% fetal bovine serum (FBS) (Hyclone, Logan, UT) and 1% penicillin-streptomycin (Mediatech, Inc., Herndon, VA) at 37°C in a humidified atmosphere of 5% CO2 in air.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from proliferating C2C12 cells using TRIzol (Invitrogen, Carlsbad, CA) according to manufacturer’s instructions. Total RNA concentration was quantified by an absorbance at 260 nm using a SmartSpec 3000 spectrophotometer (Bio-Rad, Hercules, CA). RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA) and samples with RNA integrity number (RIN) >9 were used in the array studies. Aliquots of the same RNA sample were used for all the experiments in both the microarray and qPCR-array measurements.
Label FAM
Label protocol N/A
 
Hybridization protocol N/A
Scan protocol N/A
Description Standard Taqman Assay: Quantitative real-time PCR was performed using Applied Biosystems 7900HT system and universal cycling conditions (10 min. at 95C; 15 sec. at 95C; 1 min. at 60C for 40 cycles).
Data processing Data was processed using SDS 2.3 software (Applied Biosystems, Foster City, CA). Normalized by subtracting mammu6 to get Delta CT
 
Submission date May 07, 2009
Last update date Mar 10, 2011
Contact name Jonathan Adam Gelfond
E-mail(s) gelfondjal@uthscsa.edu
Phone 210 567 0851
Organization name UT Health Science Ctr San Antonio
Department Epidemiology & Biostatistics
Lab Bioinformatics
Street address Mail Code 7933, 7703 Floyd Curl
City San Antonio
State/province TX
ZIP/Postal code 78229
Country USA
 
Platform ID GPL8527
Series (1)
GSE16000 Reproducibility of Quantitative RT-PCR Array in miRNA Expression Profiling and Comparison with Microarray Analysis

Data table header descriptions
ID_REF
VALUE Delta Ct value

Data table
ID_REF VALUE
MammU6-4395470
U87-4386735 8.834
Y1-4386739 8.318
ath-miR159a-4373390 20.395
mmu-let-7a-4373169 10.26
mmu-let-7b-4373168 9.157
mmu-let-7c-4373167 7.323
mmu-let-7d-4395394 9.153
mmu-let-7e-4395517 6.458
mmu-let-7f-4373164 10.513
mmu-let-7g-4395393 8.346
mmu-let-7i-4395332 8.298
mmu-miR-1-4395333 15.363
mmu-miR-100-4373160 8.285
mmu-miR-101a-4395364 13.455
mmu-miR-103-4373158 10.033
mmu-miR-105-4395595 12.292
mmu-miR-106a-4395589 5.292
mmu-miR-106b-4373155 9.356
mmu-miR-107-4373154 20.395

Total number of rows: 381

Table truncated, full table size 10 Kbytes.




Supplementary file Size Download File type/resource
GSM400676.txt.gz 2.9 Kb (ftp)(http) TXT
Processed data included within Sample table

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