|
| Status |
Public on Aug 31, 2022 |
| Title |
siMESH1-01_1_HG-U133A+2 |
| Sample type |
RNA |
| |
|
| Source name |
H1975 cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: H1975
transfection: plx304 vector and reverse transfeted with the siRNA targeting MESH1 CDS (siMESH1) for 72
|
| Treatment protocol |
H1975 cells were reverse transfected with the corresponding siRNA together with the Lipofectamine RNAiMAX reagent (ThermoFisher) and Opti-MEM according to the manufacture's instructions.
|
| Growth protocol |
All H1975 cells were cultured in the DMEM media supplement with 10% FBS and Penicillin/Streptomycin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted by RNAeasy kits (Qiagen) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Hybridization targets were prepared with MessageAmp™ Premier RNA Amplification Kit (Applied Biosystems/Ambion, Austin, TX) from total RNA, hybridized to GeneChip arrays in Affymetrix GeneChip® hybridization oven 645, washed in Affymetrix GeneChip® Fluidics Station 450
|
| Scan protocol |
scanned with Affymetrix GeneChip® Scanner 7G according to standard Affymetrix GeneChip® Hybridization, Wash, and Stain protocols. (Affymetrix, Santa Clara,CA).
|
| Data processing |
The data were analyzed with Agilent 2100 Bioanalyzer G2939A
|
| |
|
| Submission date |
Aug 04, 2019 |
| Last update date |
Aug 31, 2022 |
| Contact name |
Jen-Tsan Chi |
| E-mail(s) |
jentsan.chi@duke.edu
|
| Phone |
919-668-4759
|
| Organization name |
Duke University
|
| Department |
Molecular Genetics and Microbiology
|
| Street address |
101 Science Drive, DUMC 3382
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27708 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE135358 |
The transcriptional response to MESH1 silencing |
|
