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Status |
Public on Nov 04, 2019 |
Title |
Mouse 24 h RPE 4 |
Sample type |
SRA |
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Source name |
Mouse RPE/choroid
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Organism |
Mus musculus |
Characteristics |
strain: C57BL/6J gender: Male age: 30 days pmi interval: 24 h tissue: RPE/choroid
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Extracted molecule |
polyA RNA |
Extraction protocol |
Retina/RPE was isolated, stored at 4 degress in RNA later or RNA protect, respectively, and RNA was harvested miRNAeasy micro. RNA libraries were prepared for sequencing using standard Illumina protocols
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Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NextSeq 500 |
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Description |
RPE4-24_S7
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Data processing |
Raw files were aligned using RNA-STAR Rsubread's featurcounts was used to generate read counts edgeR was used for DE R enviroment was used to analyze data Genome_build: GRCm38.p6 and Panu_3.0 Supplementary_files_format_and_content: csv, raw counts from Rsubread
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Submission date |
Aug 05, 2019 |
Last update date |
Nov 04, 2019 |
Contact name |
Les Kallestad |
Organization name |
University of California Irvine
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Department |
Ophthalmology
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Lab |
Krzysztof Palczewski
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Street address |
829 Health Sciences Rd
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City |
Irvine |
State/province |
CA |
ZIP/Postal code |
92617 |
Country |
USA |
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Platform ID |
GPL19057 |
Series (1) |
GSE135415 |
Tissue- and Species-Specific Patterns of RNA metabolism in Post-Mortem Mammalian Retina and Retinal Pigment Epithelium |
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Relations |
BioSample |
SAMN12498780 |
SRA |
SRX6658310 |
Supplementary data files not provided |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
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