|
Status |
Public on Nov 04, 2019 |
Title |
Baboon 3 h RPE 4 |
Sample type |
SRA |
|
|
Source name |
Baboon RPE/choroid
|
Organism |
Papio anubis |
Characteristics |
strain: Olive Baboon gender: Female age: 11 years pmi interval: 3 h tissue: RPE/choroid
|
Extracted molecule |
polyA RNA |
Extraction protocol |
Retina/RPE was isolated, stored at 4 degress in RNA later or RNA protect, respectively, and RNA was harvested miRNAeasy micro. RNA libraries were prepared for sequencing using standard Illumina protocols
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NextSeq 500 |
|
|
Description |
THoa-RPE3_PR4-2_S8 X3hr4Aligned.out.bam
|
Data processing |
Raw files were aligned using RNA-STAR Rsubread's featurcounts was used to generate read counts edgeR was used for DE R enviroment was used to analyze data Genome_build: GRCm38.p6 and Panu_3.0 Supplementary_files_format_and_content: csv, raw counts from Rsubread
|
|
|
Submission date |
Aug 05, 2019 |
Last update date |
Nov 04, 2019 |
Contact name |
Les Kallestad |
Organization name |
University of California Irvine
|
Department |
Ophthalmology
|
Lab |
Krzysztof Palczewski
|
Street address |
829 Health Sciences Rd
|
City |
Irvine |
State/province |
CA |
ZIP/Postal code |
92617 |
Country |
USA |
|
|
Platform ID |
GPL27009 |
Series (1) |
GSE135415 |
Tissue- and Species-Specific Patterns of RNA metabolism in Post-Mortem Mammalian Retina and Retinal Pigment Epithelium |
|
Relations |
BioSample |
SAMN12498757 |
SRA |
SRX6658335 |