|
| Status |
Public on Mar 28, 2023 |
| Title |
Unstimulated vs IL-13 stimulated cells from donor #1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
neutrophils isolated from blood
|
| Organism |
Homo sapiens |
| Characteristics |
donor number: Donor 1
stimulation type: IL-13
|
| Treatment protocol |
unstimulated or stimulated with 10ng/ml IL-13
|
| Growth protocol |
Freshly isolated human neutrophils
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Qiagen RNAeasy (#74104) kit protocol
|
| Label |
Cy3
|
| Label protocol |
Nugen Ovation V2, Nugen FL-Ovation cDNA Fluorescent Module
|
| |
|
| Channel 2 |
| Source name |
neutrophils isolated from blood
|
| Organism |
Homo sapiens |
| Characteristics |
donor number: Donor 1
stimulation: Unstimulated
|
| Treatment protocol |
unstimulated or stimulated with 10ng/ml IL-13
|
| Growth protocol |
Freshly isolated human neutrophils
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Qiagen RNAeasy (#74104) kit protocol
|
| Label |
Cy5
|
| Label protocol |
Nugen Ovation V2, Nugen FL-Ovation cDNA Fluorescent Module
|
| |
|
| |
| Hybridization protocol |
Standard Agilent protocol
|
| Scan protocol |
Agilent Technologies Scanner G2505C - 5 microns per pixel
|
| Description |
Biological replicate 1 of 3. Flipped labeling.
|
| Data processing |
Agilent Feature extraction version GE2_105_Dec08
|
| |
|
| Submission date |
Aug 05, 2019 |
| Last update date |
Mar 28, 2023 |
| Contact name |
Ilkka Junttila |
| E-mail(s) |
ilkka.junttila@tuni.fi
|
| Organization name |
University of Tampere
|
| Department |
Institute of Medical and Life Science
|
| Lab |
Cytokine biology
|
| Street address |
Arvo Ylpön katu 34
|
| City |
Tampere |
| State/province |
Tampere |
| ZIP/Postal code |
33520 |
| Country |
Finland |
| |
|
| Platform ID |
GPL4133 |
| Series (1) |
| GSE135426 |
Human neutrophils stimulated with IL-13. |
|
