|
| Status |
Public on Aug 28, 2019 |
| Title |
ndl1_tassel_rep1 |
| Sample type |
SRA |
| |
|
| Source name |
5-10mm maize greenhouse-grown ndl1 mutant tassels
|
| Organism |
Zea mays |
| Characteristics |
background: B73 and Oh43
genotype: ndl1 mutant
tissue: tassel
|
| Treatment protocol |
greenhouse-grown plants from an F2 segregating population (ndl1 mutant X B73) and from inbred background OH43 were dissected and flash-frozen
|
| Growth protocol |
greenhouse-grown maize plants
|
| Extracted molecule |
total RNA |
| Extraction protocol |
samples were snap frozen in liquid nitrogen and total RNA was extracted using RNeasy kit (Qiagen) with DNAse treatment
Illumina mRNA sequencing libraries were generated using TruSeq (Illumina)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina Genome Analyzer II |
| |
|
| Data processing |
Illumina GAII software used for base calling
Supplementary_files_format_and_content: Processed data files not provided because differential expression was not performed on this data. Mapped data (i.e. bam files) was used to examine reads within a small genetic mapping window on a genome browser.
|
| |
|
| Submission date |
Aug 06, 2019 |
| Last update date |
Aug 28, 2019 |
| Contact name |
Andrea Gallavotti |
| E-mail(s) |
agallavotti@waksman.rutgers.edu
|
| Organization name |
Rutgers University
|
| Department |
Waksman Institute
|
| Lab |
Gallavotti Lab
|
| Street address |
190 Frelinghuysen Rd
|
| City |
Piscataway |
| State/province |
New Jersey |
| ZIP/Postal code |
08854-8020 |
| Country |
USA |
| |
|
| Platform ID |
GPL9361 |
| Series (1) |
| GSE135468 |
NEEDLE1 (NDL1) ENCODES A MITOCHONDRIA LOCALIZED ATPase REQUIRED FOR THERMOTOLERANT MAIZE GROWTH |
|
| Relations |
| BioSample |
SAMN12506467 |
| SRA |
SRX6660518 |
