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Sample GSM401349 Query DataSets for GSM401349
Status Public on May 12, 2009
Title Toxoplasma gondii Type I GT1 Compound 1 - 1
Sample type RNA
 
Source name Toxoplasma gondii Type I GT1 Compound 1 48hrs
Organism Toxoplasma gondii
Characteristics strain: type I strain GT1
developmental stage: bradyzoite
growth condition: grown in human foreskin fibroblasts under 3uM Compound 1 for 48hrs
Extracted molecule total RNA
Extraction protocol Parasites were purified from host cells and RNA was extracted using the Qiagen RNeasy kit (b-mercaptoethanol and DNase I treatment).
Label biotin
Label protocol Affymetrix One Cycle: 3ug starting RNA - Standard One Cycle protocol
 
Hybridization protocol Standard Affymetrix protocol with 5ug labeled cRNA.
Scan protocol Standard Affymetrix protocol
Description Type I strain GT1 grown in human foreskin fibroblasts under 3uM Compound 1 for 48hrs.
For additional Material and Methods see:
Mol Microbiol. 2008 Jun;68(6):1502-18. Epub 2008 Apr 21.
The transcription of bradyzoite genes in Toxoplasma gondii is controlled byautonomous promoter elements.
Behnke MS, Radke JB, Smith AT, Sullivan WJ Jr, White MW.
Data processing GeneSpring 7.2 RMA, Data transformation: Set measurements less than 0.01 to 0.01, Per Chip and Per Gene: Median Polishing
 
Submission date May 10, 2009
Last update date Mar 04, 2010
Contact name Michael Sean Behnke
E-mail(s) mike_behnke@yahoo.com
Organization name Washington University School of Medicine
Department Molecular Microbiology
Lab Dr David Sibley
Street address 660 S. Euclid Ave
City St Louis
State/province MO
ZIP/Postal code 63110
Country USA
 
Platform ID GPL7186
Series (1)
GSE16037 Types I GT1, II Me49, III CTGara strains - tachyzoite and Compound 1

Data table header descriptions
ID_REF
VALUE normalized data: Raw/Control
Raw RMA signal
Control Control value

Data table
ID_REF VALUE Raw Control
551.m00234_at 1.1216588 193.895 172.86452
SRS_LIKE11_at 0.62702805 8.923455 14.231348
65.m01193_at 0.8577901 872.7277 1017.41406
583.m09213_at 1.0408664 243.70682 234.13843
583.m05393_at 1.1751088 302.43268 257.36566
55.m04681_at 0.9407393 1916.7747 2037.5195
42.m03275_at 0.85086745 479.55164 563.6032
59.m03606_at 1.0665163 185.84068 174.25021
50.m03109_at 0.7914581 26.540218 33.533325
42.m07396_at 0.953557 32.32354 33.897854
AFFX-r2-Bs-lys-3_at 0.6384718 55.773315 87.35439
235.m00005_at 1.0007991 448.21466 447.8568
49.m03242_at 1.005711 148.98628 148.14026
541.m01162_at 0.979375 30.782318 31.430573
49.m00059_at 1.112302 103.381874 92.94407
20.m00005_at 1.1139591 15457.052 13875.78
49.m03297_at 1.0135292 19.433916 19.174501
50.m05652_at 0.74479765 27.313307 36.672115
83.m01182_at 1.5013214 107.624466 71.68649
38.m02375_at 1.0246886 21.560122 21.040657

Total number of rows: 9045

Table truncated, full table size 375 Kbytes.




Supplementary file Size Download File type/resource
GSM401349.CEL.gz 947.6 Kb (ftp)(http) CEL
Processed data included within Sample table

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