NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM401352 Query DataSets for GSM401352
Status Public on May 12, 2009
Title Toxoplasma gondii Type II Me49 tachyzoite - 2
Sample type RNA
 
Source name Type II Me49 tachyzoite
Organism Toxoplasma gondii
Characteristics strain: type II strain Me49
developmental stage: tachyzoite
growth condition: grown in human foreskin fibroblasts
Extracted molecule total RNA
Extraction protocol Parasites were purified from host cells and RNA was extracted using the Qiagen RNeasy kit (b-mercaptoethanol and DNase I treatment).
Label biotin
Label protocol Affymetrix One Cycle: 3ug starting RNA - Standard One Cycle protocol
 
Hybridization protocol Standard Affymetrix protocol with 5ug labeled cRNA.
Scan protocol Standard Affymetrix protocol
Description Type II strain Me49 tachyzoites grown in human foreskin fibroblasts.
For additional Material and Methods see:
Mol Microbiol. 2008 Jun;68(6):1502-18. Epub 2008 Apr 21.
The transcription of bradyzoite genes in Toxoplasma gondii is controlled byautonomous promoter elements.
Behnke MS, Radke JB, Smith AT, Sullivan WJ Jr, White MW.
Data processing GeneSpring 7.2 RMA, Data transformation: Set measurements less than 0.01 to 0.01, Per Chip and Per Gene: Median Polishing
 
Submission date May 10, 2009
Last update date Mar 04, 2010
Contact name Michael Sean Behnke
E-mail(s) mike_behnke@yahoo.com
Organization name Washington University School of Medicine
Department Molecular Microbiology
Lab Dr David Sibley
Street address 660 S. Euclid Ave
City St Louis
State/province MO
ZIP/Postal code 63110
Country USA
 
Platform ID GPL7186
Series (1)
GSE16037 Types I GT1, II Me49, III CTGara strains - tachyzoite and Compound 1

Data table header descriptions
ID_REF
VALUE normalized data: Raw/Control
Raw RMA signal
Control Control value

Data table
ID_REF VALUE Raw Control
551.m00234_at 0.9981521 120.25818 120.48081
SRS_LIKE11_at 0.79878265 7.9229465 9.918776
65.m01193_at 1.0566329 749.2623 709.1037
583.m09213_at 0.92272174 150.5759 163.18669
583.m05393_at 0.7079882 126.9956 179.37529
55.m04681_at 0.9955778 1413.8035 1420.0833
42.m03275_at 0.95982975 377.0333 392.81265
59.m03606_at 0.91626394 111.27713 121.44659
50.m03109_at 0.91749245 21.443275 23.37161
42.m07396_at 1.0464429 24.722921 23.625675
AFFX-r2-Bs-lys-3_at 1.0597078 64.518295 60.8831
235.m00005_at 0.92483777 288.68005 312.1413
49.m03242_at 1.0064253 103.91223 103.248825
541.m01162_at 6.0989227 133.60338 21.906063
49.m00059_at 0.9486321 61.451366 64.77892
20.m00005_at 1.2991676 12564.193 9670.957
49.m03297_at 0.86628306 11.576998 13.363989
50.m05652_at 0.93073434 23.788864 25.559242
83.m01182_at 0.44234547 22.10095 49.963097
38.m02375_at 0.9381554 13.757708 14.664637

Total number of rows: 9045

Table truncated, full table size 375 Kbytes.




Supplementary file Size Download File type/resource
GSM401352.CEL.gz 911.7 Kb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap
External link. Please review our privacy policy.