|
| Status |
Public on Aug 08, 2019 |
| Title |
WT H2O2 versus wt SD |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
ACY044 in SD
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: ACY044
treatment: none
|
| Treatment protocol |
select cell cultures were treated with 0.4M KCl or 0.4mM H2O2 for 20min
|
| Growth protocol |
Cells were grown to log phase in synthetic complete medium
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Cells were lysed in hot phenol and the polyA RNA purified using oligoDT cellulose
|
| Label |
Cy3
|
| Label protocol |
mRNA was reverse transcribed incooperatigin aa-UTP and then labelled with Cy5 or Cy3
|
| |
|
| Channel 2 |
| Source name |
ACY044 in SD
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: ACY044
treatment: 0.4mM H2O2, 20min
|
| Treatment protocol |
select cell cultures were treated with 0.4M KCl or 0.4mM H2O2 for 20min
|
| Growth protocol |
Cells were grown to log phase in synthetic complete medium
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Cells were lysed in hot phenol and the polyA RNA purified using oligoDT cellulose
|
| Label |
Cy5
|
| Label protocol |
mRNA was reverse transcribed incooperatigin aa-UTP and then labelled with Cy5 or Cy3
|
| |
|
| |
| Hybridization protocol |
Hybridization was performed using Agilent Hyb buffers and chambers following the manufactures suggested protocols
|
| Scan protocol |
Arrays were scanned on a Genepix 300b scanner
|
| Data processing |
Arrays were analyzed using GenePix 6.1, and then LOWESS normalized
|
| |
|
| Submission date |
Aug 07, 2019 |
| Last update date |
Aug 08, 2019 |
| Contact name |
Andrew Capaldi |
| E-mail(s) |
capaldi@email.arizona.edu
|
| Organization name |
Univ of Arizona
|
| Department |
MCB
|
| Street address |
1007 E Lowell St
|
| City |
Tucson |
| State/province |
AZ |
| ZIP/Postal code |
85721 |
| Country |
USA |
| |
|
| Platform ID |
GPL16244 |
| Series (1) |
| GSE135546 |
The InsP7 phosphatase Siw14 regulates levels of inositol pyrophosphates and affects activity of general stress transcription factors Msn2 and Msn4 |
|
