|
Status |
Public on Aug 10, 2019 |
Title |
LPS2nuc bulk RNA-seq |
Sample type |
SRA |
|
|
Source name |
brain
|
Organism |
Mus musculus |
Characteristics |
tissue: brain sampletype: Microglia nuclei sequencing protocol: 3’ Quantseq tissue type: fresh treatment: LPS
|
Treatment protocol |
I.p. PBS or LPS injection, 1mg/kg for 3 hours
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA isolation with Rneasy Plus Micro Kit (Qiagen) Quant Seq 3’ mRNA-Seq Library Prep Kit FWD (Lexogen) and Chromium Single Cell 3’ v2 kit from 10x Genomics
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NextSeq 500 |
|
|
Description |
BE180802_Lexogen_086_S80_R1_001
|
Data processing |
QC with FASTQC Trimming with TrimGalore v0.4.5 Alignment with HiSat v2.1 Counting with FeatureCounts Supplementary_files_format_and_content: Raw count file
|
|
|
Submission date |
Aug 09, 2019 |
Last update date |
Aug 10, 2019 |
Contact name |
Emma Gerrits |
E-mail(s) |
e.gerrits@rug.nl, e.gerrits@umcg.nl
|
Organization name |
UMCG
|
Department |
BSCS
|
Lab |
Molecular Neurobiology
|
Street address |
Antonius Deusinglaan 1
|
City |
Groningen |
State/province |
Groningen |
ZIP/Postal code |
9713AV |
Country |
Netherlands |
|
|
Platform ID |
GPL19057 |
Series (1) |
GSE135618 |
Transcriptional profiling of microglia; current state of the art and future perspectives |
|
Relations |
BioSample |
SAMN12542156 |
SRA |
SRX6686196 |