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| Status |
Public on Aug 14, 2019 |
| Title |
C-AO-MBR (SS) |
| Sample type |
SRA |
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| Source name |
Suspended sludge
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| Organism |
wastewater metagenome |
| Characteristics |
sample type: Suspended sludge
mbr system: Conventional
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| Extracted molecule |
total RNA |
| Extraction protocol |
Fast-DNA SPIN kit for soil (MP Biomedicals, Solon, OH, USA).
RNA libraries were prepared for sequencing using standard Illumina protocols.
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| Library strategy |
OTHER |
| Library source |
transcriptomic |
| Library selection |
other |
| Instrument model |
Illumina MiSeq |
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| Description |
Bacteria
Sample 6
processed data file: Classification.txt
processed data file: C-AO-MBR_SS_S6.report.pdf
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| Data processing |
Sequencing was carried out by using Illumina MiSeq sequencing on an Illumina MiSeq device (Illumina Inc., San Diego, CA, USA) with 600 cycles (300 cycles for each paired read and 12 cycles for the barcode sequence) according to the manufacturer’s instructions.
Sequence analysis was conducted using the 16S-based metagenomics workflow of MiSeq Reporter version 2.6.2.3 (Illumina).
Samples were gathered into a single library for sequencing on Illumina MiSeq sequencing system which generated paired 300 bp reads.
Sequences were then demultiplexed based on index sequences.
Genome_build: V3 and V4 regions of the 16S rRNA gene.
Supplementary_files_format_and_content: Classification.txt: Tab-delimited text file includes Sample number 4 (BF-AO-MBR (SP)), Sample number 5 (BF-AO-MBR (SS)) and Sample number 6 (C-AO-MBR (SS)).
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| Submission date |
Aug 13, 2019 |
| Last update date |
Aug 14, 2019 |
| Contact name |
Dome Adoonsook |
| E-mail(s) |
dome_fb@hotmail.com
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| Phone |
0899562526
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| Organization name |
Dome
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| Street address |
Suthep
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| City |
muang |
| State/province |
ChiangMai |
| ZIP/Postal code |
50100 |
| Country |
Thailand |
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| Platform ID |
GPL27052 |
| Series (1) |
| GSE135797 |
Comparison of microbial communities between conventional MBR system and biofilm MBR system |
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| Relations |
| BioSample |
SAMN12567847 |
| SRA |
SRX6709908 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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