Microarrays were hybridized with Cy3- and Cy5-labeled probe pairs of Ubi1::CBF3 and nontransgenic plants under drought stress condision. Transgenic and nontransgenic rice (Oryza sativa cv Nakdong) seeds were germinated on soil and grown in the greenhouse (16-h-light/8-h-dark cycles). Transgenic and NC plants were treated for 2 h with drought (air-dry) at the greenhouse continuous light. Total RNA (100 ¥ìg) was prepared from leaf tissues of 14-day-old transgenic and nontransgenic seedlings (5-10 plants each) and the mRNA was purified from total RNAs using Qiagen oligotex column (Qiagen, USA). Preparation of fluorescence labeled probes and microarray hybridization was performed as procedures provided by Genisphere 3DNA Array Detection Array 50 Kit (Version 2) (Genisphere, USA). The microarray was scanned with Genepix 5.0B (Axon Instruments, USA) and the quality of chip data was analysed with statistical R language and sma package in Bioconductor (http://www.bioconductor.org/)implemented on Linux platform. Multivariate statistics was performed with Acuity 3.1 (Axon Instruments, USA). Keywords = Abiotic stress, CBF3
