|
| Status |
Public on Jun 03, 2009 |
| Title |
SLN:1620 - B - mAdbID:38689 |
| Sample type |
RNA |
| |
|
| Source name |
SLN:1620
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Lymph node
disease state: Follicular Lymphoma
individual: SLN:1620
clinical info: Follow up status: dead
clinical info: Follow up years: 9.36
clinical info: Stage >2: yes
clinical info: LDH ratio >1: yes
clinical info: Number of extranodal sites >1: yes
clinical info: Age >60: no
clinical info: IPI score: 2
clinical info: Immune response 1: 9.14
clinical info: Immune response 2: 8.35
clinical info: With translocation: NA
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
FastTrack 2.0 mRNA Isolation Kit
Extraction method: oligo dt cellulose
Other: mRNA was extracted using the FastTrack 2.0 mRNA Isolation Kit according to manufacturer's instructions (Invitrogen, Carlsbad, CA).
|
| Label |
biotin
|
| Label protocol |
Affymetrix Labeling Protocol
Other: Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 microg mRNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Affymetrix Hybridization Protocol
Other: Following fragmentation, 20 micrograms of cRNA were hybridized for 16 hours at 45C on U133A/B GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
Affy Scanning Protocol
Other: Scanning was performed by the Affymetrix 3000 Scanner.
|
| Description |
mAdb experiment ID: 38689
|
| Data processing |
MAS 5.0 Data Processing Protocol
Calculation Method: The data were analyzed with Microarray Suite version 5.0 (MA S 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
May 15, 2009 |
| Last update date |
Jun 03, 2009 |
| Contact name |
Louis M. Staudt |
| E-mail(s) |
lstaudt@mail.nih.gov
|
| Phone |
301-402-1892
|
| Organization name |
National Cancer Institute
|
| Department |
Lymphoid Malignancies Branch
|
| Lab |
Louis M Staudt
|
| Street address |
9000 Rockville Pike, Bldg 10, Rm 4N114
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL97 |
| Series (1) |
| GSE16131 |
Differences Between Follicular Lymphoma With and Without Translocation t(14;18) |
|
