|
| Status |
Public on Aug 20, 2019 |
| Title |
4T1-GFP-fLuc cells from MFP tumor #2 on day 28, bio rep2 (from sample 'Breast Tumor2-4wks') |
| Sample type |
RNA |
| |
|
| Source name |
4T1-GFP-fLuc cells isolated from MFP tumor #2 on day 28
|
| Organism |
Mus musculus |
| Characteristics |
strain: balb/c
|
| Treatment protocol |
No cell lines were exposed to any type of treatment.
|
| Growth protocol |
Cells were grown using standard tissue culture until 60-80% confluent at which point RNA was isolated.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from the cells using the Quick RNA MiniPrep Zymo Research Kit (Genesee scientific).
|
| Label |
biotin
|
| Label protocol |
The biotin-labeled cDNA was prepared from 250 ng of total RNA using the Ambion WT Expression Kit and Affymetrix WT Terminal Labeling Kit.
|
| |
|
| Hybridization protocol |
Fragmented and biotin-labeled cDNA at a concentration of 25 ng/ul or 2.25 ug total was hybridized to the Mouse Gene 2.1 ST peg plate. The Mouse Gene 2.1 ST peg plate was hybridized, washed, stained and scanned using the Affymetrix GeneTitan MC instrument. The default method for the Mouse Gene 2.1 peg plate was used on the GeneTitan. The hybridization time for the expression plates was 17 hours and the temperature was 48 C.
|
| Scan protocol |
The Mouse Gene 2.1 ST peg plate was scanned using the Affymetrix GeneTitan MC instrument.
|
| Description |
4T1-GFP-fLuc cells isolated from MFP tumor #2 on day 28, biological rep2 (belonging to sample type 'Breast Tumor2-4wks')
Gene expression data from 4T1-GFP-fLuc (murine breast cancer cells labeled with Green Fluorescent Protein and firefly luciferase) that were injected into mammary fat pad and then isolated on day 28 after injection
|
| Data processing |
The data were analyzed using Affymetrix Expression Console and the algorithm used was RMA (with background adjustment, quantile normalization and summarization).
|
| |
|
| Submission date |
Aug 19, 2019 |
| Last update date |
Aug 20, 2019 |
| Contact name |
Alessandro Porrello |
| E-mail(s) |
alessandro.porrello@gmail.com
|
| Organization name |
University of North Carolina at Chapel Hill
|
| Department |
Lineberger Comprehensive Cancer Center
|
| Street address |
450 West Drive
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27599 |
| Country |
USA |
| |
|
| Platform ID |
GPL17400 |
| Series (1) |
| GSE136031 |
Expression data from 4T1 subclones derived from mammary fat pad tumors (MFP), axillary lymph node tumors (AxLN), and axillary lymph node-derived lung metastases (AxLN-LuM) |
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