|
| Status |
Public on Aug 20, 2019 |
| Title |
4T1-mCherry-rLuc cells from AxLN-derived lung metastasis #1 on day 43, bio rep1 (from sample 'AxLN Lung Mets2-6wks') |
| Sample type |
RNA |
| |
|
| Source name |
4T1-mCherry-rLuc cells isolated from AxLN-derived lung metastasis #1 on day 43
|
| Organism |
Mus musculus |
| Characteristics |
strain: balb/c
|
| Treatment protocol |
No cell lines were exposed to any type of treatment.
|
| Growth protocol |
Cells were grown using standard tissue culture until 60-80% confluent at which point RNA was isolated.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from the cells using the Quick RNA MiniPrep Zymo Research Kit (Genesee scientific).
|
| Label |
biotin
|
| Label protocol |
The biotin-labeled cDNA was prepared from 250 ng of total RNA using the Ambion WT Expression Kit and Affymetrix WT Terminal Labeling Kit.
|
| |
|
| Hybridization protocol |
Fragmented and biotin-labeled cDNA at a concentration of 25 ng/ul or 2.25 ug total was hybridized to the Mouse Gene 2.1 ST peg plate. The Mouse Gene 2.1 ST peg plate was hybridized, washed, stained and scanned using the Affymetrix GeneTitan MC instrument. The default method for the Mouse Gene 2.1 peg plate was used on the GeneTitan. The hybridization time for the expression plates was 17 hours and the temperature was 48 C.
|
| Scan protocol |
The Mouse Gene 2.1 ST peg plate was scanned using the Affymetrix GeneTitan MC instrument.
|
| Description |
4T1-mCherry-rLuc cells isolated from AxLN-derived lung metastasis #1 on day 43, biological rep1 (belonging to sample type 'AxLN Lung Mets2-6wks')
Gene expression data from 4T1-mCherry-rLuc (murine breast cancer cells labeled with mCherry fluorescent reporter and renilla luciferase) that were injected into axillary lymph nodes and then isolated from a lung metastasis on day 43 after injection
|
| Data processing |
The data were analyzed using Affymetrix Expression Console and the algorithm used was RMA (with background adjustment, quantile normalization and summarization).
|
| |
|
| Submission date |
Aug 19, 2019 |
| Last update date |
Aug 20, 2019 |
| Contact name |
Alessandro Porrello |
| E-mail(s) |
alessandro.porrello@gmail.com
|
| Organization name |
University of North Carolina at Chapel Hill
|
| Department |
Lineberger Comprehensive Cancer Center
|
| Street address |
450 West Drive
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27599 |
| Country |
USA |
| |
|
| Platform ID |
GPL17400 |
| Series (1) |
| GSE136031 |
Expression data from 4T1 subclones derived from mammary fat pad tumors (MFP), axillary lymph node tumors (AxLN), and axillary lymph node-derived lung metastases (AxLN-LuM) |
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