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Sample GSM404000 Query DataSets for GSM404000
Status Public on May 16, 2009
Title CD4TESTJS#1
Sample type RNA
 
Source name spleen
Organism Mus musculus
Characteristics age: 6 weeks
genetic background: 4+ 8- B220-
phenotype markers: 4+8-B220-
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol Glass slide teasing Flow sorting
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description CD4 Test JS#1
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date May 15, 2009
Last update date May 24, 2016
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 18.0059
10344622 256.111
10344624 301.116
10344633 766.9825
10344637 173.1565
10344653 15.8394
10344658 165.1101
10344674 13.5973
10344679 42.2212
10344707 257.7134
10344713 401.4404
10344723 322.4299
10344725 40.154
10344741 2067.1273
10344743 31.7918
10344750 383.8869
10344772 25.7097
10344789 105.5055
10344797 382.4192
10344799 150.2757

Total number of rows: 24922

Table truncated, full table size 422 Kbytes.




Supplementary file Size Download File type/resource
GSM404000_EA07068_82676_MoGene_CD4TESTJS#1.CEL.gz 4.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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