|
| Status |
Public on Aug 26, 2022 |
| Title |
pPV-MF2 |
| Sample type |
RNA |
| |
|
| Source name |
CD34+ cells
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: bone marrow (BM)
genotype: JAK2V617F+
age: 43
Sex: F
diagnosis: pPV-MF
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Blood and bone marrow mononuclear cells from MPN patients were isolated using Ficoll density gradient centrifugation. Enrichment of the CD34+ cell population was performed by magnetic cell separation (MACS) with the CD34 MicroBead Kit, human (Miltenyi). RNA was isolated using the RNeasy Micro Kit (QIAGEN).
|
| Label |
Biotin
|
| Label protocol |
Fragmented, biotinylated cDNAs were prepared according according to the Affymetrix Gene Chip WT Pico Reagent Kit according to the manufactorers instructions: input each 6.6 ng total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 5.5 µg of fragmented biotin labelled cDNAs were hybridized for 16 hrs at 45°C and 60 rpm on HTA 2.0 ST Arrays. Gene Chips were washed and stained in the Affymetrix Fluidics Station 450 using the Fluidics Program FS450_0001.
|
| Scan protocol |
Gene Chips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Data processing |
Raw image data were analysed with Affymetrix® Expression Console™ Software (Affymetrix, USA), and gene expression intensities were normalized with SST-RMA algorithm (Affymetrix).
|
| |
|
| Submission date |
Aug 26, 2019 |
| Last update date |
Aug 26, 2022 |
| Contact name |
Bernd Denecke |
| E-mail(s) |
bernd.denecke@rwth-aachen.de
|
| Phone |
+49 241 8089918
|
| Organization name |
RWTH Aachen University
|
| Department |
IZKF Aachen
|
| Street address |
Pauwelsstrasse 30
|
| City |
Aachen |
| State/province |
NRW |
| ZIP/Postal code |
52074 |
| Country |
Germany |
| |
|
| Platform ID |
GPL17586 |
| Series (1) |
| GSE136335 |
Gene expression data from CD34+ BMMCs/PBMCs from JAK2V617F positive MPN patients vs. Healthy controls |
|
