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Status |
Public on Aug 26, 2022 |
Title |
PV8 |
Sample type |
RNA |
|
|
Source name |
CD34+ cells
|
Organism |
Homo sapiens |
Characteristics |
tissue: bone marrow (BM) genotype: JAK2V617F+ age: 57 Sex: F diagnosis: PV
|
Extracted molecule |
total RNA |
Extraction protocol |
Blood and bone marrow mononuclear cells from MPN patients were isolated using Ficoll density gradient centrifugation. Enrichment of the CD34+ cell population was performed by magnetic cell separation (MACS) with the CD34 MicroBead Kit, human (Miltenyi). RNA was isolated using the RNeasy Micro Kit (QIAGEN).
|
Label |
Biotin
|
Label protocol |
Fragmented, biotinylated cDNAs were prepared according according to the Affymetrix Gene Chip WT Pico Reagent Kit according to the manufactorers instructions: input each 6.6 ng total RNA
|
|
|
Hybridization protocol |
Following fragmentation, 5.5 µg of fragmented biotin labelled cDNAs were hybridized for 16 hrs at 45°C and 60 rpm on HTA 2.0 ST Arrays. Gene Chips were washed and stained in the Affymetrix Fluidics Station 450 using the Fluidics Program FS450_0001.
|
Scan protocol |
Gene Chips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
Data processing |
Raw image data were analysed with Affymetrix® Expression Console™ Software (Affymetrix, USA), and gene expression intensities were normalized with SST-RMA algorithm (Affymetrix).
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|
|
Submission date |
Aug 26, 2019 |
Last update date |
Aug 26, 2022 |
Contact name |
Bernd Denecke |
E-mail(s) |
bernd.denecke@rwth-aachen.de
|
Phone |
+49 241 8089918
|
Organization name |
RWTH Aachen University
|
Department |
IZKF Aachen
|
Street address |
Pauwelsstrasse 30
|
City |
Aachen |
State/province |
NRW |
ZIP/Postal code |
52074 |
Country |
Germany |
|
|
Platform ID |
GPL17586 |
Series (1) |
GSE136335 |
Gene expression data from CD34+ BMMCs/PBMCs from JAK2V617F positive MPN patients vs. Healthy controls |
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