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Sample GSM4047085 Query DataSets for GSM4047085
Status Public on May 05, 2021
Title Muscle_young_biological rep10
Sample type RNA
 
Source name vastus lateralis, young
Organism Homo sapiens
Characteristics gender: male
age (y): 24.9
disease state: healthy
tissue: vastus lateralis muscle
Growth protocol Vastus lateralis muscle biopsy from men
Extracted molecule total RNA
Extraction protocol Vastus lateralis biopsies were snap-frozen in liquid N2. RNA was isolated using a TissueRuptor and RNeasy Fibrous Tissue Mini Kit (Qiagen). Integrity of RNA was verified using an Agilent Bioanalyser with the RNA 6000 Nano labchip® kit (Agilent). Concentration and purity was determined with a Nanodrop ND-1000 spectrophotometer (Nanodrop Technologies). The depletion of the ribosomal fractions was processed using 2 µg of total RNA and magnetic beads from Ribominus Kit (Invitrogen).
Label biotin
Label protocol Biotinylated single stranded cDNA were prepared from 400 ng of depleted total RNA according to the Affymetrix WT protocol (Ambion WT Expression kit).
 
Hybridization protocol Following fragmentation and terminal labeling, 5.5 µg of single stranded cDNA were hybridized for 16 h at 45°C and 60 rpm on GeneChip® Human Exon 1.0 ST Array in the Affymetrix Oven. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 with Hybridization Wash and Stain Affymetrix kit.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
Data processing Data were generated with Affymetrix Expression Console v 1.2.1 software using robust multiarray average (RMA) algorithm. Affymetrix data files were processed using GeneSpring GX 14.9 13 (Agilent ).
 
Submission date Aug 26, 2019
Last update date May 05, 2021
Contact name Daniel Bechet
E-mail(s) daniel.bechet@inrae.fr
Phone 33473624178
Organization name INRA
Department UNH
Lab Proteostasis
Street address Theix
City Saint Genes Champanelle
ZIP/Postal code 63122
Country France
 
Platform ID GPL5175
Series (1)
GSE136344 Muscle transcriptomic profiling of chronological aging and metabolic syndrome in men

Data table header descriptions
ID_REF
VALUE Quantile normalized gene level expression values from GeneSpring

Data table
ID_REF VALUE
2315373 41.853554
2315554 125.71178
2315633 147.77188
2315674 106.42388
2315739 104.42594
2315894 167.69818
2315918 50.573807
2315951 186.38724
2316069 121.052574
2316218 142.30077
2316245 63.264217
2316379 223.5332
2316558 479.23312
2316605 103.43312
2316746 180.03574
2316905 80.97024
2316953 98.27531
2317246 88.68036
2317317 104.27433
2317434 572.95465

Total number of rows: 15940

Table truncated, full table size 274 Kbytes.




Supplementary file Size Download File type/resource
GSM4047085_20100623_35J_3592_HuEx-1_0-st-v2_.CEL.gz 22.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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