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Sample GSM4047097 Query DataSets for GSM4047097
Status Public on May 05, 2021
Title Muscle_Old_biological rep11
Sample type RNA
 
Source name vastus lateralis, old
Organism Homo sapiens
Characteristics gender: male
age (y): 73.4
disease state: healthy
tissue: vastus lateralis muscle
Growth protocol Vastus lateralis muscle biopsy from men
Extracted molecule total RNA
Extraction protocol Vastus lateralis biopsies were snap-frozen in liquid N2. RNA was isolated using a TissueRuptor and RNeasy Fibrous Tissue Mini Kit (Qiagen). Integrity of RNA was verified using an Agilent Bioanalyser with the RNA 6000 Nano labchip® kit (Agilent). Concentration and purity was determined with a Nanodrop ND-1000 spectrophotometer (Nanodrop Technologies). The depletion of the ribosomal fractions was processed using 2 µg of total RNA and magnetic beads from Ribominus Kit (Invitrogen).
Label biotin
Label protocol Biotinylated single stranded cDNA were prepared from 400 ng of depleted total RNA according to the Affymetrix WT protocol (Ambion WT Expression kit).
 
Hybridization protocol Following fragmentation and terminal labeling, 5.5 µg of single stranded cDNA were hybridized for 16 h at 45°C and 60 rpm on GeneChip® Human Exon 1.0 ST Array in the Affymetrix Oven. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 with Hybridization Wash and Stain Affymetrix kit.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
Data processing Data were generated with Affymetrix Expression Console v 1.2.1 software using robust multiarray average (RMA) algorithm. Affymetrix data files were processed using GeneSpring GX 14.9 13 (Agilent ).
 
Submission date Aug 26, 2019
Last update date May 05, 2021
Contact name Daniel Bechet
E-mail(s) daniel.bechet@inrae.fr
Phone 33473624178
Organization name INRA
Department UNH
Lab Proteostasis
Street address Theix
City Saint Genes Champanelle
ZIP/Postal code 63122
Country France
 
Platform ID GPL5175
Series (1)
GSE136344 Muscle transcriptomic profiling of chronological aging and metabolic syndrome in men

Data table header descriptions
ID_REF
VALUE Quantile normalized gene level expression values from GeneSpring

Data table
ID_REF VALUE
2315373 46.54113
2315554 185.62334
2315633 167.17288
2315674 139.82538
2315739 156.0984
2315894 224.2527
2315918 56.855217
2315951 197.83978
2316069 171.90434
2316218 315.9009
2316245 75.44253
2316379 238.71017
2316558 478.3683
2316605 134.86377
2316746 204.08047
2316905 123.40318
2316953 129.8025
2317246 101.07164
2317317 139.92761
2317434 575.9918

Total number of rows: 15940

Table truncated, full table size 274 Kbytes.




Supplementary file Size Download File type/resource
GSM4047097_20100622_32A_3561_HuEx-1_0-st-v2_.CEL.gz 22.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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