|
| Status |
Public on Nov 01, 2019 |
| Title |
fetal biceps_control_140d_ replicate 6 |
| Sample type |
RNA |
| |
|
| Source name |
fetal biceps_control_140d_ replicate 6
|
| Organism |
Ovis aries |
| Characteristics |
tissue: fetal biceps femoris
treatment group: control 140d gestation
|
| Treatment protocol |
Ewes were infused with 1mg/kg/day cortisol or vehicle from approximately day 115 to 140 d of pregnancy (n=7, cortisol; n=7 control). Fetal Samples of left ventriclar free wall (n=5 control, n=5 cortisol) were then collected and RNA extracted.
|
| Growth protocol |
Continuous infusions of cortisol to the ewe were begun at approximately day 115 of gestation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol (Life Technologies, Carlsbad, CA), and purified through Qiagen Rneasy+ kits with on-column DNase digestion (QIAGEN, Valencia, CA) according to manufacturers' protocols.Total RNA concentration was measured by Nanodrop ND-1000 and RNA quality was monitored by Agilent Bioanalyzer
|
| Label |
Cy3
|
| Label protocol |
Cyanine-3 (Cy3) labeled cRNA was prepared from 500 ng RNA using the One-Color Quick Amp Labeling Kit (Agilent) according to the manufacturer's instructions, followed by RNAeasy column purification (QIAGEN, Valencia, CA). Dye incorporation and cRNA yield were checked with the NanoDrop ND-1000 Spectrophotometer.
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| |
|
| Hybridization protocol |
600 ng of Cy3-labelled cRNA (specific activity >8.3 pmol Cy3/µg cRNA) was fragmented and hybridized to Agilent Sheep Oligo Microarrays (G4813A) by the ICBR facility at the University of Florida according to Agilent's methodology.
|
| Scan protocol |
Slides were scanned on the Agilent DNA Microarray Scanner (G2505B) using one color (green) scan setting for 8x15k array slides, by the ICBR facility at University of Florida.
|
| Description |
Gene expression at 140 days gestation in fetal biceps femoris
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 10.1.1.1 (Agilent) using default parameters (protocol GE1-v5_10 and Grid: 019921_D_F_20100112) background detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded. Data were normalized to the chip median and log transformed.
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| |
|
| Submission date |
Aug 27, 2019 |
| Last update date |
Nov 02, 2019 |
| Contact name |
Maureen Keller-Wood |
| E-mail(s) |
kellerwd@cop.ufl.edu
|
| Organization name |
University of Florida
|
| Department |
Pharmacodynamics
|
| Lab |
Keller-Wood
|
| Street address |
1225 Center Dr
|
| City |
Gainesville |
| State/province |
Florida |
| ZIP/Postal code |
32610 |
| Country |
USA |
| |
|
| Platform ID |
GPL10427 |
| Series (2) |
| GSE136384 |
Effect of ~ 25 days cortisol treatment on late gestation fetal biceps femoris |
| GSE136385 |
Effect of ~ 25 days cortisol treatment on late gestation fetal |
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