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| Status |
Public on Aug 29, 2019 |
| Title |
NAT1 |
| Sample type |
SRA |
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| Source name |
pancreas
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| Organism |
Homo sapiens |
| Characteristics |
tissue: normal pancreas
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| Extracted molecule |
total RNA |
| Extraction protocol |
PDAC or normal adjacent tissues were removed, flash frozen in liquid nitrogen and total RNA was extracted using Trizol reagent. The ribosomal RNA was removed using the Ribozero series kit and linear RNA was digested using RNaseR.
RNA libraries were prepared for sequencing using standard illumina protocols.
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
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| Data processing |
Illumina Casava1.7 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to whole genome（version:hg38）using STAR（version:2.5）.
The circRNAs were identified using CIRI（version:2.0.5）and find_circ（version:1.2）.Quantity of gene-expression level was estimated by Reads Per Billion Mapped Reads (RPB).
Genome_build: hg38
Supplementary_files_format_and_content: tab-delimited text files include RPB values for each sample.
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| Submission date |
Aug 28, 2019 |
| Last update date |
Aug 29, 2019 |
| Contact name |
Yuming Luo |
| Organization name |
Sun yat-sen Memorial Hospital
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| Department |
General Surgery
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| Street address |
107 Yanjiang West Avenue,Yuexiu district
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| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510120 |
| Country |
China |
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| Platform ID |
GPL20795 |
| Series (1) |
| GSE136569 |
circNFIB suppresses lymphatic metastasis of pancreatic cancer |
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| Relations |
| BioSample |
SAMN12647838 |
| SRA |
SRX6767063 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM4051660_N25581.txt.gz |
297.9 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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