|
| Status |
Public on Sep 30, 2019 |
| Title |
emu_FL_LPM_8 |
| Sample type |
SRA |
| |
|
| Source name |
Embryonic tissue
|
| Organism |
Dromaius novaehollandiae |
| Characteristics |
tissue: Undifferentiated lateral plate mesoderm at the forelimb level
Stage: HH10
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using PicoPure columns
libraries were synthesized from 50ng of total RNA by automation using Kappa reagents followed by 15 cycles of replication.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
RNA-seq reads were mapped using STAR to reference genomes (galGal4 or droNov1)
Quantification via FeatureCounts
Differential gene expression was determined using DESeq.
Genome_build: galGal4 for chick or droNov1 for emu
Supplementary_files_format_and_content: Tab delimited .txt files with transcript counts generated from featureCounts
|
| |
|
| Submission date |
Sep 03, 2019 |
| Last update date |
Sep 30, 2019 |
| Contact name |
John Young |
| E-mail(s) |
jyoung@genetics.med.harvard.edu
|
| Organization name |
Harvard Medical School
|
| Department |
Genetics
|
| Lab |
Tabin
|
| Street address |
77 Avenue Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL27417 |
| Series (2) |
| GSE136774 |
Attenuated FGF signaling underlies the forelimb heterochrony in the emu Dromaius novaehollandiae (RNA-seq data) |
| GSE136776 |
Attenuated FGF signaling underlies the forelimb heterochrony in the emu Dromaius novaehollandiae |
|
| Relations |
| BioSample |
SAMN12684367 |
| SRA |
SRX6793597 |
