|
Status |
Public on Mar 26, 2021 |
Title |
Biopsy patient2 lesional skin day 14 |
Sample type |
RNA |
|
|
Source name |
Lesional skin punch biopsy day 14
|
Organism |
Homo sapiens |
Characteristics |
patient diagnosis: psoriasis tissue: skin gender: Male disease state (ls/nl): LS day: 14
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA from skin biopsies was isolated using the SV Total RNA Isolation System according to the manufacturer's instructions.
|
Label |
Biotin
|
Label protocol |
Biotinylated cRNA was prepared according to the protocol for the human Clariom D assay (ThermoFisher Scientific).
|
|
|
Hybridization protocol |
Following fragmentation, cRNA was hybridized for 16 hr at 45C on the human Clariom D GeneChip according to the manufacturer's instructions.
|
Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 system.
|
Description |
Gene expression data from lesional psoriatic skin at day 14
|
Data processing |
The microarray data were summarized applying the SST-RMA (signal space transformation - robust multi-array average) gene level method as implemented in the Transcriptome Analysis Console (TAC) 4.0 software (ThermoFisher Scientific).
|
|
|
Submission date |
Sep 10, 2019 |
Last update date |
Mar 27, 2021 |
Contact name |
Thomas Litman |
E-mail(s) |
tlitman@hotmail.com
|
Phone |
4541851753
|
Organization name |
University of Copenhagen
|
Department |
ISIM
|
Street address |
Blegdamsvej 3C
|
City |
Copenhagen N |
State/province |
Outside the US or Canada |
ZIP/Postal code |
2200 |
Country |
Denmark |
|
|
Platform ID |
GPL23126 |
Series (2) |
GSE137218 |
Skin expression data from psoriasis patients treated with secukinumab |
GSE137221 |
Skin and blood expression data from psoriasis patients treated with secukinumab |
|