|
| Status |
Public on Sep 17, 2019 |
| Title |
Buccal swab, 100973330053_R06C02 |
| Sample type |
genomic |
| |
|
| Source name |
buccal swab genomic DNA
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: buccal epithelial cells
age: 0.22739726
gender: M
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted using the Gentra Puregene Buccal Cell Kit (Qiagen #158845) and DNA was bisulfite converted using the EZ DNA Methylation Kit™ (Zymo Research).
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
Bisulfite converted DNA was amplified, fragmented and hybridized to Illumina Infinium HumanMethylation450k Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged with BeadArray Reader using standard recommended Illumina scanner setting
|
| Description |
NA
|
| Data processing |
GenomeStudio software V2011.1 with GenomeStudio Methylation Analysis Module Version 1.9.1000 (background-subtraction, color-correction)
|
| |
|
| Submission date |
Sep 16, 2019 |
| Last update date |
Sep 17, 2019 |
| Contact name |
Michael S. Kobor |
| Organization name |
Centre for Molecular Medicine and Therapeutics / University of British Columbia
|
| Department |
Medical Genetics
|
| Lab |
Kobor
|
| Street address |
950 West 28th Avenue
|
| City |
Vancouver |
| State/province |
BC |
| ZIP/Postal code |
V5Z 4H4 |
| Country |
Canada |
| |
|
| Platform ID |
GPL13534 |
| Series (2) |
| GSE137495 |
The PedBE clock estimates DNA methylation age in pediatric buccal cells [APrON] |
| GSE137503 |
The PedBE clock accurately estimates DNA methylation age in pediatric buccal cells |
|
