|
| Status |
Public on Sep 19, 2020 |
| Title |
n2_LK32_B |
| Sample type |
RNA |
| |
|
| Source name |
N2, S Basal (liquid), LK32, 8h
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
media: S Basal
treatment: LK32
tissue: Whole organism
strain: N2
|
| Treatment protocol |
Animals were grown until young adult stage. Then they were washed off the plates into 15 mL conicals, rinsed 2 times in S Basal, supplemented with E. coli OP50 for foos, and dispensed into 6-well plates, 6,000 worms/well. LK molecules at 100 uM final or corresponding amount of DMSO solvent control were added to wells.
|
| Growth protocol |
N2 worms were synchronized by hypochlorite treatment. Arrested L1s were plated on 10 cm NGM plates seeded with E. coli OP50 and grown 56 h at 22°C until they reached young adult stage.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using TRI Reagent (Molecular Research Center) according to manufacturer's instructions and purified using an RNeasy column (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Affymetrix standard protocol
|
| |
|
| Hybridization protocol |
Affymetrix standard protocol
|
| Scan protocol |
Affymetrix standard protocol
|
| Data processing |
Data were analyzed with GC-RMA in the R programming language, using the Bioconductor package gcrma. Analysis was performed using default settings.
|
| |
|
| Submission date |
Sep 16, 2019 |
| Last update date |
Sep 20, 2020 |
| Contact name |
Natalia V Kirienko |
| E-mail(s) |
kirienko@rice.edu
|
| Organization name |
Rice University
|
| Department |
BioSciences
|
| Lab |
Kirienko
|
| Street address |
6100 Main St. MS140
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77005 |
| Country |
USA |
| |
|
| Platform ID |
GPL200 |
| Series (1) |
| GSE137516 |
Novel Immune Modulators Enhance Caenorhabditis elegans Resistance to Multiple Pathogens |
|
