|
| Status |
Public on Oct 18, 2019 |
| Title |
101070190020_R05C01 |
| Sample type |
genomic |
| |
|
| Source name |
buccal swab genomic DNA
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Buccal epithelial cells
age: 32.6406570841889
gender: Female
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted using the Masterpure system (Epicentre, USA) and quantified using a NanoPhotometer P300 (Implen, Germany). Bisulfite converted using the EZ DNA Methylation Kit™ (Zymo Research).
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol.
|
| |
|
| Hybridization protocol |
Bisulfite converted DNA was amplified, fragmented and hybridized to Illumina Infinium HumanMethylation450k Beadchip using standard Illumina protocol.
|
| Scan protocol |
Arrays were imaged with BeadArray Reader using standard recommended Illumina scanner setting.
|
| Data processing |
Minfi preprocessIllumina was used to process samples (for background subtraction and colour correction).
Average Beta (after minfi::preprocessIllumina in R).
|
| |
|
| Submission date |
Sep 18, 2019 |
| Last update date |
Oct 18, 2019 |
| Contact name |
Michael S. Kobor |
| Organization name |
Centre for Molecular Medicine and Therapeutics / University of British Columbia
|
| Department |
Medical Genetics
|
| Lab |
Kobor
|
| Street address |
950 West 28th Avenue
|
| City |
Vancouver |
| State/province |
BC |
| ZIP/Postal code |
V5Z 4H4 |
| Country |
Canada |
| |
|
| Platform ID |
GPL13534 |
| Series (2) |
| GSE137503 |
The PedBE clock accurately estimates DNA methylation age in pediatric buccal cells |
| GSE137688 |
The PedBE clock accurately estimates DNA methylation age in pediatric buccal cells [dataset 15, MAVAN mothers cohort] |
|
